Reduce amplification bias caused by variable miRNA abundance using automated PCR normalization for NEXTFLEX small RNA sequencing workflows.
In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.
CLONTECH's SMART RACE cDNA amplification process diagram On your mark, get set, go! And the RACE is on to obtain that potentially elusive full-length cDNA for characterizing the best gene ever. In the ...